Stem Cell Heterogeneity: Methods and Protocols (Methods in Molecular Biology)

Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity. This goes against the long-held tenet that stem cells, defined by their capacity for self-renewal and lineage development, comprised a homogenous population, thus providing the reader with a new avenue of exploration into the complex world of stem cell study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stem Cell Heterogeneity: Methods and Protocols serves as an ideal guide for investigators exploring this important area of research.    1. The Heterogeneity of Stem Cells: A Brief Overview             Gyrgyi Muzes and Ferenc Sipos   2. Establishment and Characterization of Nave Pluripotency in Human Embryonic Stem Cells             Sharat Warrier, Mina Popovic, Margot Van der Jeught, and Bjrn Heindryckx   3. An Effective and Reliable Xeno-Free Cryopreservation Protocol for Single Human Pluripotent Stem Cells             Guoliang Meng, Anna Poon, Shiying Liu, and Derrick E. Rancourt   4. Clonal Analysis of Cells with Cellular Barcoding: When Numbers and Sizes Matter             Leonid V. Bystrykh and Mirjam E. Belderbos   5. Analysis of Cell Cycle Status of Murine Hematopoietic Stem Cells             Krzysztof Szade, Karolina Bukowska-Strakova, Monika Zukowska, Alicja Jozkowicz, and Jzef Dulak   6. Dissecting Transcriptional Heterogeneity in Pluripotency: Single Cell Analysis of Mouse Embryonic Stem Cells             Ana M.V. Guedes, Domingos Henrique, and Elsa Abranches   7. Generation of Regionally Specific Neural Progenitor Cells (NPCs) and Neurons from Human Pluripotent Stem Cells (hPSCs)             Josh Cutts, Nicholas Brookhouser, and David. A Brafman   8. Isolation and Culture of Embryonic Stem Cells, Mesenchymal Stem Cells, and Dendritic Cells from Humans and Mice             Srabani Kar, Shinjini Mitra, and Ena Ray Banerjee   9. Decoding the Epigenetic Heterogeneity of Human Pluripotent Stem Cells with Seamless Gene Editing             Amar M. Singh, Dustin W. Perry, Valeriya V. Adjan Steffey, Kenneth Miller, and Daniel W. Allison   10. Stencil Micropatterning for Spatial Control of Human Pluripotent Stem Cell Fate Heterogeneity             Jun Yuan, Geetika Sahni, and Yi-Chin Toh   11. Visualizing the Functional Heterogeneity of Muscle Stem Cells             Yasuo Kitajima, Shizuka Ogawa, and Yusuke Ono   12. Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies             Chiara Franzin, Martina Piccoli, Luca Urbani, Carlo Biz, Piergiorgio Gamba, Paolo De Coppi, and Michela Pozzobon   13. Measuring ATP Concentration in a Small Number of Murine Hematopoietic Stem Cells             Krzysztof Szade, Monika Zukowska, Alicja Jozkowicz, and Jzef Dulak   14. Growth Factor-Free Pre-Vascularization of Cell Sheets for Tissue Engineering             Marina Costa, Rogrio P. Pirraco, Mariana T. Cerqueira, Rui L. Reis, and Alexandra P. Marques   15. In Vitro Culture of Human Hematopoietic Stem Cells in Serum Free Medium and Their Monitoring by Flow Cytometry             Marc Cloutier, Christine Jobin, Carl Simard, and Sonia Nron   16. Aerosol-Based Cell Therapy for Treatment of Lung Diseases             Badrul Hisham Yahaya, Egi Kardia, and Nur Shuhaidatul Sarmiza Abdul Halim   17. Induction of Inner Ear Hair Cells from Mouse Embryonic Stem Cells In Vitro             Masahide Yoshikawa and Yukiteru Ouji   18. Maintenance of Dermal Papilla Cells by Wnt-10b In Vitro             Yukiteru Ouji and Masahide Yoshikawa   19. Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro             Yukiteru Ouji and Masahide Yoshikawa   20. Enzyme-Free Dissociation of Neurospheres by a Microfluidic Chip-Based Method             Ching-Hui Lin, Hao-Chen Chang, and Chia-Hsien Hsu   21. Automated Cell-Based Quantitation of 8-OHdG Damage             Bilge Debelec-Butuner, Aykut Bostanci, Lisa Heiserich, Caroline Eberle, Filiz Ozcan, Mutay Aslan, Dirk Roggenbuck, and Kemal Sami Korkmaz   22. CoCl2 Administration to Vascular MSC Cultures as an In Vitro Hypoxic System to Study Stem Cell Survival and Angiogenesis             Carmen Ciavarella, Silvia Fittipaldi, and Gianandrea Pasquinelli   23. Reporter Systems to Study Cancer Stem Cells             Caner Saygin, Mohamed Samour, Anastasia Chumakova, Awad Jarrar, Justin D. Lathia, and Ofer Reizes   24. Agent-Based Modeling of Cancer Stem Cell Driven Solid Tumor Growth             Jan Poleszczuk, Paul Macklin, and Heiko Enderling   25. Induction of a Tumor-Metastasis-Receptive Microenvironment as an Unwanted Side Effect After Radio/Chemotherapy and In Vitro and In Vivo Assays to Study This Phenomenon             Gabriela Schneider, Zachariah Payne Sellers, and Mariusz Z. Ratajczak   26. Isolation and Propagation of Glioma Stem Cells from Acutely Resected Tumors             Jin-Kyu Jung, Mark R. Gilbert, and Deric M. Park   27. Isolation and Characterization of Cancer Stem Cells of the Non-Small Cell Lung Cancer (A549) Cell Line             Nazilah Abdul Satar, Norashikin Zakaria, Noor Hanis Abu Halim, and Badrul Hisham Yahaya