Mesenchymal Stem Cells: Methods and Protocols (Methods in Molecular Biology)

For over forty years, mesenchymal stem cells (MSCs) have been scrutinized and studied, garnering much attention due to their broad therapeutic efficacy. In this essential book, leaders in the field were assembled to contribute detailed methodologies for the isolation and characterization of human and rodent MSCs. Cutting edge and easy to use, this book is the perfect resource for scientists attempting to pursue this important and ever-developing field of research. Table of Contents: Preface. Darwin J. Prockop, Donald G. Phinney, Bruce A. Bunnell Part I: Isolation of human multipotential stromal cells from bone marrow and adipose tissue. Chapter 1. Isolation and culture of bone marrow-derived human multipotent stromal cells (hMSCs). Margaret Wolfe, Radhika Pochampally, William Swaney and Roxanne Reger Chapter 2. Mesenchymal stem cells from adult bone marrow. Mark F. Pittenger Chapter 3. A method to isolate and purify human bone marrow stromal stem cells. Stan Gronthos and Andrew C. W. Zannettino Chapter 4. Adipose-derived stem cells. John K. Fraser, Min Zhu, Isabella Wulur and Zeni Alfonso Chapter 5. Isolation of human adipose-derived stem cells from biopsies and liposuction specimens. Severine G Dubois, Z. Elizabeth Floyd, Sanjin Zvonic, Gail Kilroy, Xiying Wu, Stacy Carling, Yuan Di C. Halvorsen, Eric Ravussin and Jeffrey M Gimble Part II: Manipulation of human multipotential stromal cells. Chapter 6. Colony forming unit assays for MSCs. Radhika Pochampally Chapter 7. Differentiation and characterization of human MSCs. Roxanne L. Reger, H. Alan Tucker, and Margaret R. Wolfe Chapter 8. Freezing harvested hMSCs and recovery of hMSCs from frozen vials for subsequent expansion, analysis and experimentation. Roxanne L. Reger and Margaret R. Wolfe Chapter 9. Gene expression analysis at the single cell level using the human bone marrow stromal cell as a model: sample preparation methods. Beerelli Seshi Chapter 10. Assays of MSCs with microarrays. Joni Ylstalo, Radhika Pochampally and Darwin J. Prockop Chapter 11. Gene delivery to mesenchymal stem cells. Reza Izadpanah and Bruce A. Bunnell Part III: Isolation and characterization of murine multipotential stromal cells from bone marrow. Chapter 12. Isolation of mesenchymal stem cells from murine bone marrow by Immunodepletion. Donald G. Phinney