Advanced Fluorescence Reporters in Chemistry and Biology III: Applications in Sensing and Imaging (Springer Series on Fluorescence)

The key element of any fluorescence sensing or imaging technology is the fluorescence reporter, which transforms the information on molecular interactions and dynamics into measurable signals of fluorescence emission. This book, written by a team of frontline researchers, demonstrates the broad field of applications of fluorescence reporters, starting from nanoscopic properties of materials, such as self-assembled thin films, polymers and ionic liquids, through biological macromolecules and further to living cell, tissue and body imaging. Basic information on obtaining and interpreting experimental data is presented and recent progress in these practically important areas is highlighted. The book is addressed to a broad interdisciplinary audience. Part I   General aspects  Interfacial behavior of fluorescent dyes. Power and weakness of nanoscopic description Alexander P. Demchenko and Semen O. Yesylevskyy  Part II   Probing condensed media   Fluorescence probing of physicochemical characteristics of the room temperature ionic liquids Anunay Samanta  Fluorescence spectroscopy in polymer science Tanzeela N. Raja and Albert M. Brouwer  Fluorescence probing in structurally anisotropic materials. From liquid crystals to macromolecules, micelles and lipid bilayers Semen O. Yesylevskyy and Alexander P. Demchenko Part III   Fluorescence reporters in biosensing Optimized dyes for protein and nucleic acid detection Sergiy M.Yarmoluk, Vladyslava B. Kovalska, Kateryna D.Volkova  Functional nucleic acids for fluorescence based biosensing applications Jennifer Lee, Lawrence Lin, and Yingfu Li  Part IV   Cell imaging with organic dyes  Covalent labeling of biomolecules in living cells Tilman Plass and Carsten Schultz  Tetracysteine and bipartite tags for biarsenical organic fluorophores Carla Spagnuolo, Maria Joselevich, Federico Coluccio Leskow and Elizabeth A. Jares-Erijman  Labeling of oligohistidine-tagged proteins Jacob Piehler  Part V   Tissue and whole body imaging  In vivo imaging of vascular targets using near-infrared fluorescent probes Jan Klohs and Markus Rudin  Whole-body imaging of hematopoietic and cancer cells using near infrared probes Vyacheslav Kalchenko, Michal Neeman and Alon Harmelin